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Smears were stained with Eosin Y (Leica Biosystems Richmond Inc., Buffalo Grove, IL, USA) and spermatozoa with the following morphologies were considered to be abnormal: (i) headless, (ii) hookless, (iii) double headed, (iv) broken tail, (v) coiled tail, and (vi) double tailed.27,28 Testicular morphological evaluation The excised testes were fixed in 10% formalin and kept at room temperature overnight