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Treatment with 5-amino-1mq upregulates proliferation‑related genes (Ki67, PCNA), promotes cell cycle progression, and improves cell fusion efficiency to form more multinucleated myotubes, achieving functional levels similar to those of young mice.Additionally, 5-amino-1mq inhibits muscle stem cell apoptosis, reduces oxidative stress damage, and maintains stemness

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In future, we plan to use the RNAi (RNA interference) method both at the cellular and animal levels to further investigate in-depth about the mechanism, especially the network between ER stress and the NO system